He continued to work with chromatography in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll, carotenes, and xanthophylls.Since these components have different colors (green, orange, and yellow, respectively) they gave the technique its name. Hydrophobic Interaction Chromatography: Fundamentals and Applications in Biomedical Engineering 605 Fig. These groups can range from methyl, ethyl, propyl, octyl, or phenyl groups. Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity.

In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase.

Hydrophobic interaction chromatography 2. In this type of chromatography, hydrophobic groups such as phenyl, octyl, or butyl, are attached to the stationary column.

(A) The basic structure of a HIC resin is depicted, and a protein is schematized highli ghting the hydrophobic zones on the protein surface. Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another. phenomena form the basis for hydrophobic interaction chromatography (HIC). Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules by using the function of hydrophobicity. HIC is used in the purification of proteins over a broad range of scales-in both analytical and preparatory scale applications. Hydrophobic interaction chromatography (HIC) is a valuable tool used in protein purification applications. Categories Instrumentation Tags Affinity chromatography, Chromatography, Chromatography Applications, Chromatography Principle, Chromatography Types, Column chromatography, Dye-ligand chromatography, Gas chromatography (GS), Gel-permeation (molecular sieve) chromatography, High-pressure liquid chromatography (HPLC), Hydrophobic interaction chromatography, Ion-exchange chromatography… • Different basis of separation • Weaker interactions → Less structural damage → Maintain high activity 3.

Title: Recent Advancement in Application of Hydrophobic Interaction Chromatography for Aggregate Removal in Industrial Purification Process VOLUME: 10 ISSUE: 4 Author(s):Yuefeng Lu, Brian Williamson and Ronald Gillespie Affiliation:Global Process Engineering, Process Development, Amgen Inc., Thousand Oaks, CA 91320, USA. Alternatives • Gel filtration chromatography • Ion exchange chromatography • Reverse phase chromatography Why HIC? 1. Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another.

Hydrophobic interaction chromatography (HIC) is an analytical technique that utilizes the hydrophobic properties of molecules to achieve their separation 1. Protein retention mechanism in HIC. In this type of chromatography, sample molecules with hydrophobic portion(s) interact with and bind to the hydrophobic groups (e.g., phenyl groups) attached to the column stationary phase. Hydrophobic-Interaction Membrane Chromatography HIC is used to purify molecules based on differences in their hydrophobicity.

It was first described by Shepard and Tiselius in 1949 using the term “salting out chromatography.”